Synonymous Codon Context

Since the revelation of the genetic code in 1961 by Crick et al. it has been understood that the interaction between a specific codon in the mRNA being translated, and the three bases that define the anticodon of the cognate aminoacyl-tRNA determines the decoding process Since the revelation of the genetic code in 1961 by Crick et al. it has been understood that the interaction between a specific codon in the mRNA being translated, and the three bases that define the anticodon of the cognate aminoacyl-tRNA determines the decoding process.

Currently, analysis of a codon sequence of a gene has focused on the frequency a particular code is used for an organism. Additionally, state-of- the-art DNA analysis regards synonymous codons as irrelevant or unknown for classification. We now know that synonymous mutations are not evolutionarily silent. Rather these changes affect translation speed and accuracy, co-translational folding, protein secretion and overall expression levels.

T3S Technologies has developed the capability to finely-tune gene expression to dramatically increase protein production by several folds without the use of plasmids or altering the native protein sequence. 

If you are interested in our sequence optimization services please contact us.